prikaz prve stranice dokumenta Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis
  Download
PDF 235.5 KB
Scientific paper - Original scientific paper
Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis
Collegium antropologicum, 35 (2011), 1; 93-100. urn:nbn:hr:239:481797
Pavić, Roman; Pavić, Michele L.; Tvrdeić, Ante; Tot, Ozana Katarina; Heffer, Marija

Cite this document

Pavić, R., Pavić, M. L., Tvrdeić, A., Tot, O. K. & Heffer, M. (2011). Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis. Collegium antropologicum, 35. (1), 93-100. Retrieved from https://urn.nsk.hr/urn:nbn:hr:239:481797

Pavić, Roman, et al. "Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis." Collegium antropologicum, vol. 35, no. 1, 2011, pp. 93-100. https://urn.nsk.hr/urn:nbn:hr:239:481797

Pavić, Roman, Michele L. Pavić, Ante Tvrdeić, Ozana Katarina Tot and Marija Heffer. "Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis." Collegium antropologicum 35, no. 1 (2011): 93-100. https://urn.nsk.hr/urn:nbn:hr:239:481797

Pavić, R., et al. (2011) 'Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis', Collegium antropologicum, 35(1), pp. 93-100. Available at: https://urn.nsk.hr/urn:nbn:hr:239:481797 (Accessed 27 May 2023)

Pavić R, Pavić ML, Tvrdeić A, Tot OK, Heffer M. Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis. Collegium antropologicum [Internet]. 2011 [cited 2023 May 27];35(1):93-100. Available at: https://urn.nsk.hr/urn:nbn:hr:239:481797

R. Pavić, M. L. Pavić, A. Tvrdeić, O. K. Tot and M. Heffer, "Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis", Collegium antropologicum, vol. 35, no. 1, pp. 93-100, 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:239:481797. [Accessed: 27 May 2023]

Please login to the repository to save this object to your list.
Metadata
Title (english)Rat Sciatic Nerve Crush Injury and Recovery Tracked by Plantar Test and Immunohistochemistry Analysis
Title (croatian)Ozljeda nagnječenjem ishijadičnog živca kod štakora i praćenje oporavka istog putem plantarnog testa i imunohistokemijskim putem
AuthorRoman Pavić
MBZ: 285214
AuthorMichele L. Pavić
AuthorAnte Tvrdeić
MBZ: 157335
AuthorOzana Katarina Tot
MBZ: 344381
AuthorMarija Heffer
MBZ: 178985
Author's institution(Clinical hospital center Osijek)
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Neurology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Abstract (english)
An experimental crush injury to the sciatic nerve, with a crush force of 49.2 N (pressure p=1.98x108 Pa), was inflicted in 30 male rats (Wistar). A control group (sham), with the same number of rats, was also operated upon exactly as the experimental group but without the crush injury. We tested the sensory and motor recovery of the sciatic nerve with Hargreaves method, using an apparatus from Ugo Basile, Italy. Testing was continued for both legs of each rat, injured and uninjured, starting preoperatively (0 day), and then 1, 7, 14, 21, and 28 days postoperatively. The same experiment was run simultaneously with the sham group. The Plantar test showed recovery of the sensory and motor function of the sciatic nerve, though not complete recovery, by 28 days. An immunohistochemical experiment was run in parallel with the plantar test on L3-L6 segments of the spinal cord from where the sciatic nerve extends. We used antibodies for Myelin-associated glycoprotein (MAG), and gangliosides GD1a and GT1b on the aforesaid part of the spinal cord. The immunohistochemical methods showed changes in sensory and motor axons in the spinal cord segment L3-L6 which suggest correspondence with the results of the Plantar test, in terms of recovery of the sensory and motor function after injury of the sciatic nerve. The immunohistochemical results also show ipsilateral and contralateral changes following injury. Results of the plantar test are suggestive that the rat shows compensation for an injury in its contralateral leg.
Abstract (croatian)
Na 30 muških štakora (Wistar) učinjeno je eksperimentalno ozljeđivanje ishijadičnog živca nagnječenjem, upotrebnom sile od 49,2 N (tlak p=1.98x108 Pa). Također je na jednakom broju štakora učinjen kontrolni dio pokusa (sham) gdje je prikazan neozlijeđeni ishijadični živac uz prethodno razmicanje muskulature. Iza toga smo ispitivali osjetilni i motorički oporavak ozlijeđenog živca upotrebom Hargreavesove metode plantarnog testa, uz upotrebu aparata Ugo Basile, Italija. Testiranje je vršeno kontinuirano za obje noge štakora, ozlijeđenu i neozlijeđenu, prijeoperacijski (0 dan), prvi dan nakon ozljede, 7, 14, 21 i 28 dan poslije ozljede. Istovjetno je učinjeno i za kontrolnu skupinu životinja (sham). Plantarni test je pokazao oporavak motoričke i osjetilne funkcije ozlijeđenog ishijadičnog živca 28 dan ali nije u potpunosti dosegao prijeoperacijske vrijednosti. Paralelno sa plantarnim testom radili smo imunohistokemijski pokus na dijelu kralježnične moždine L3-L6, gdje je polazište ishijadičnog živca. Koristili smo protutijela na mijelinom udruženi glikoprotein (MAG), i gangliozide GD1a i GT1b u navedenom dijelu kralježnične moždine. Imunohistokemijskom metodom pokazali smo promjene na motoričkim i osjetilnim aksonima u kralježničnom dijelu L3-L6 i usporedili sa rezultatima plantarnog testa, u smislu oporavaka motorike i osjeta nakon ozljede ishijadičnog živca. Imunohistokemijskom analizom dobiveni rezultati također pokazuju ipsilateralne i kontralateralne promjene nakon ozljede. Rezultati plantarnog testa pokazuju motoričku kompenzaciju ozlijeđene noge štakora neozlijeđenom.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCollegium antropologicum
Numberingvol. 35, no. 1, pp. 93-100
p-ISSN0350-6134
URN:NBNurn:nbn:hr:239:481797
Publication2011
Document URLhttps://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=96070
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-03-27 09:32:11