prikaz prve stranice dokumenta Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
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Scientific paper - Original scientific paper
Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
International Journal of Environmental Research and Public Health, 14 (2017), 1; 1-13. https://doi.org/10.3390/ijerph14010109
Kizivat, Tomislav; Smolić, Martina; Marić, Ivana; Tolušić Levak, Maja; Smolić, Robert; Bilić Ćurčić, Ines; Kuna, Lucija; Mihaljević, Ivan; Včev, Aleksandar; Tucak-Zorić, Sandra

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Kizivat, T., Smolić, M., Marić, I., Tolušić Levak, M., Smolić, R., Bilić Ćurčić, I. ... Tucak Zorić, S. (2017). Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis. International Journal of Environmental Research and Public Health, 14. (1), 1-13. doi: 10.3390/ijerph14010109

Kizivat, Tomislav, et al. "Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis." International Journal of Environmental Research and Public Health, vol. 14, no. 1, 2017, pp. 1-13. https://doi.org/10.3390/ijerph14010109

Kizivat, Tomislav, Martina Smolić, Ivana Marić, Maja Tolušić Levak, Robert Smolić, Ines Bilić Ćurčić, Lucija Kuna, Ivan Mihaljević, Aleksandar Včev and Sandra Tucak Zorić. "Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis." International Journal of Environmental Research and Public Health 14, no. 1 (2017): 1-13. https://doi.org/10.3390/ijerph14010109

Kizivat, T., et al. (2017) 'Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis', International Journal of Environmental Research and Public Health, 14(1), pp. 1-13. doi: 10.3390/ijerph14010109

Kizivat T, Smolić M, Marić I, Tolušić Levak M, Smolić R, Bilić Ćurčić I, and sur.. Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis. International Journal of Environmental Research and Public Health [Internet]. 2017 [cited 2024 December 08];14(1):1-13. doi: 10.3390/ijerph14010109

T. Kizivat, et al., "Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis", International Journal of Environmental Research and Public Health, vol. 14, no. 1, pp. 1-13, 2017. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:239:713480. [Accessed: 08 December 2024]

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Metadata
Title (english)Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
AuthorTomislav Kizivat
MBZ: 302955
AuthorMartina Smolić
MBZ: 292155
AuthorIvana Marić
AuthorMaja Tolušić Levak
MBZ: 338680
AuthorRobert Smolić
MBZ: 292166
AuthorInes Bilić Ćurčić
MBZ: 268184
AuthorLucija Kuna
MBZ: 353546
AuthorIvan Mihaljević
AuthorAleksandar Včev
MBZ: 141721
AuthorSandra Tucak-Zorić
MBZ: 320540
Author's institution(Clinical hospital center Osijek)
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Urology
Abstract (english)
Urolithiasis is characterized by the formation and retention of solid crystals within the urinary tract. Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. The aim of the study is to explore possible effects of antioxidant pre-treatment on inhibition of oxidative stress. Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. Oxidative stress was induced by exposure of cells to sodium oxalate in concentration of 8 mM. In order to prevent oxidative stress, cells were pre-treated with three different concentrations of l-arginine and vitamin E. Oxidative stress was evaluated by determining the expression of superoxide dismutase (SOD), osteopontin (OPN), and by the concentration of glutathione (GSH). In all three cell lines, pre-treatment of antioxidants increased cell survival. Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. Our results indicate that an antioxidant pre-treatment with l-arginine and vitamin E is able to hamper oxalate-induced oxidative stress in kidney epithelial cells and as such could play a role in prevention of urolithiasis
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Environmental Research and Public Health
Numberingvol. 14, no. 1, pp. 1-13
e-ISSN1660-4601
DOIhttps://doi.org/10.3390/ijerph14010109
URN:NBNurn:nbn:hr:239:713480
Publication2017
Document URLhttps://www.mdpi.com/1660-4601/14/1/109
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2019-10-29 09:17:18