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Scientific paper - Original scientific paper
PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses
Collegium antropologicum, 36 (2012), 2; 79-82. urn:nbn:hr:239:307829
Pauzar, Biljana; Karner, Ivan; Glavaš-Obrovac, Ljubica; Štefanić, Mario; Dmitrović, Branko

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Pauzar, B., Karner, I., Glavaš Obrovac, Lj., Štefanić, M. & Dmitrović, B. (2012). PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses. Collegium antropologicum, 36. (2), 79-82. Retrieved from https://urn.nsk.hr/urn:nbn:hr:239:307829

Pauzar, Biljana, et al. "PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses." Collegium antropologicum, vol. 36, no. 2, 2012, pp. 79-82. https://urn.nsk.hr/urn:nbn:hr:239:307829

Pauzar, Biljana, Ivan Karner, Ljubica Glavaš Obrovac, Mario Štefanić and Branko Dmitrović. "PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses." Collegium antropologicum 36, no. 2 (2012): 79-82. https://urn.nsk.hr/urn:nbn:hr:239:307829

Pauzar, B., et al. (2012) 'PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses', Collegium antropologicum, 36(2), pp. 79-82. Available at: https://urn.nsk.hr/urn:nbn:hr:239:307829 (Accessed 17 November 2024)

Pauzar B, Karner I, Glavaš Obrovac Lj, Štefanić M, Dmitrović B. PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses. Collegium antropologicum [Internet]. 2012 [cited 2024 November 17];36(2):79-82. Available at: https://urn.nsk.hr/urn:nbn:hr:239:307829

B. Pauzar, I. Karner, Lj. Glavaš Obrovac, M. Štefanić and B. Dmitrović, "PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses", Collegium antropologicum, vol. 36, no. 2, pp. 79-82, 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:239:307829. [Accessed: 17 November 2024]

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Metadata
Title (english)PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses
Title (croatian)Onkogen PAX8-PPARg u folikularnim tumorima štitnjače – RT-PCR i imunohistokemijska analiza
AuthorBiljana Pauzar
MBZ: 209910
AuthorIvan Karner
MBZ: 103355
AuthorLjubica Glavaš-Obrovac
MBZ: 162671
AuthorMario Štefanić
MBZ: 288502
AuthorBranko Dmitrović
MBZ: 130004
Author's institution(Clinical hospital center Osijek)
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Oncology
Abstract (english)
US-guided fine needle aspiration cytology is currently the best diagnostic tool for thyroid nodules. However, it is not sensitive and specific enough for differentiating between benign and malignant follicular tumors. A potentially useful marker for this differentiation is the PAX8-PPARg rearrangement, identified in follicular thyroid carcinomas, but not in follicular adenomas or other types of thyroid tumors. The aim of this research was to determine the clinical significance of the PAX8-PPARg oncogene in diagnostics follicular thyroid tumors. The study included 62 patients with follicular or Hürthle cell tumors. Gene expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) from paraffin embedded tissues, and PCR products were checked using the agarose gel electrophoresis. The immunohistochemical analysis was performed on archive paraffin embedded tissues with the monoclonal PPARã antibody. The statistical analysis has indicated that neither the expression of PAX8-PPARg mRNA, nor the immunohystochemical analysis with the PPARg antibody correlate with the patohystological diagnosis. The oncogene PAX8-PPARg has not met the expectations as a reliable tumor marker for differentiation between benign and malignant thyroid tumors, which makes the only reliable histological criteria – capsular and vascular invasion.
Abstract (croatian)
Ciljana citološka punkcija pod kontrolom ultrazvuka metoda je izbora u u dijagnostici čvorastih promjena, no nedovoljno osjetljiva i specifična za razlikovanje benignih i malignih folikularnih tumora. Potencijalno koristan marker za njihovu diferencijaciju je onkogen PAX8-PPARã, identificiran kod folikularnog karcinoma, a ne kod folikularnog adenoma ni drugih tumora štitnjače. Cilj ovog istra`ivanja je određivanje kliničkog značaja PAX8-PPARã gena u dijagnostici folikularnih tumora štitnjače. U istraživanje su uključena 62 ispitanika s folikularnim ili Hürthleovim tumorom. Ekspresija gena je određena metodom lančane reakcije polimeraze s obrnutom transkripcijom (RT-PCR) iz parafinskih rezova, a PCR produkti provjereni elektroforezom na agaroznom gelu. Na arhivskim parafinskim rezovima je izvršeno imunohistokemijsko bojanje monoklonalnim protutijelom PPARã. Ekspresija PAX8-PPARg mRNA, kao ni imunohistokemijska analiza PPARg protutijelom ne korelira s patohistološkom dijagnozom. Onkogen PAX8-PPARg nije opravdao očekivanja u smislu pouzdanog tumorskog markera koji bi omogućio diferencijaciju benignih i malignih tumora štitnjače, te je i dalje jedino pouzdan histološki kriterij – proboj kapsule i vaskularna.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCollegium antropologicum
Numberingvol. 36, no. 2, pp. 79-82
p-ISSN0350-6134
URN:NBNurn:nbn:hr:239:307829
Publication2012
Document URLhttps://hrcak.srce.hr/96460
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-05-19 09:09:41