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Scientific paper - Original scientific paper
Differential Expression of TFF Genes and Proteins in Breast Tumors
Acta clinica Croatica, 57 (2018), 2; 264-277. https://doi.org/10.20471/acc.2018.57.02.06
Levak Tolušić, Maja; Mihalj, Martina; Koprivčić, Ivan; Novak, Sanja; Bijelić, Nikola; Baus Lončar, Mirela; Belovari, Tatjana; Kralik, Kristina; Pauzar, Biljana

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Levak Tolušić, M., Mihalj, M., Koprivčić, I., Novak, S., Bijelić, N., Baus Lončar, M. ... Pauzar, B. (2018). Differential Expression of TFF Genes and Proteins in Breast Tumors. Acta clinica Croatica, 57. (2), 264-277. doi: 10.20471/acc.2018.57.02.06

Levak Tolušić, Maja, et al. "Differential Expression of TFF Genes and Proteins in Breast Tumors." Acta clinica Croatica, vol. 57, no. 2, 2018, pp. 264-277. https://doi.org/10.20471/acc.2018.57.02.06

Levak Tolušić, Maja, Martina Mihalj, Ivan Koprivčić, Sanja Novak, Nikola Bijelić, Mirela Baus Lončar, Tatjana Belovari, Kristina Kralik and Biljana Pauzar. "Differential Expression of TFF Genes and Proteins in Breast Tumors." Acta clinica Croatica 57, no. 2 (2018): 264-277. https://doi.org/10.20471/acc.2018.57.02.06

Levak Tolušić, M., et al. (2018) 'Differential Expression of TFF Genes and Proteins in Breast Tumors', Acta clinica Croatica, 57(2), pp. 264-277. doi: 10.20471/acc.2018.57.02.06

Levak Tolušić M, Mihalj M, Koprivčić I, Novak S, Bijelić N, Baus Lončar M, and sur.. Differential Expression of TFF Genes and Proteins in Breast Tumors. Acta clinica Croatica [Internet]. 2018 [cited 2024 December 10];57(2):264-277. doi: 10.20471/acc.2018.57.02.06

M. Levak Tolušić, et al., "Differential Expression of TFF Genes and Proteins in Breast Tumors", Acta clinica Croatica, vol. 57, no. 2, pp. 264-277, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:239:933099. [Accessed: 10 December 2024]

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Metadata
Title (english)Differential Expression of TFF Genes and Proteins in Breast Tumors
Title (croatian)Izražaj TFF gena i proteina u tumorima dojke
AuthorMaja Levak Tolušić
MBZ: 338680
AuthorMartina Mihalj
MBZ: 322792
AuthorIvan Koprivčić
MBZ: 311933
AuthorSanja Novak
MBZ: 326355
AuthorNikola Bijelić
MBZ: 313935
AuthorMirela Baus Lončar
MBZ: 222563
AuthorTatjana Belovari
MBZ: 209063
AuthorKristina Kralik
AuthorBiljana Pauzar
MBZ: 209910
Author's institution(Clinical hospital center Osijek)
Author's institutionJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Surgery
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Oncology
Abstract (english)
The objective of this study was to determine diff erential expression of TFF1, TFF2 and TFF3 genes and proteins in breast tumor subtypes. In addition, we investigated the correlation between TFF genes within tumor subgroups, and TFF genes with clinical and pathologic characteristics of the tumor. Study group included 122 patients with surgically removed breast tumors. Samples were investigated using qRT-PCR and immunohistochemistry. TFF1 and TFF3 genes and proteins were expressed in breast tumors, while the levels of TFF2 gene and protein expression were very low or undetectable. TFF1 was signifi cantly more expressed in benign tumors, while TFF3 was more expressed in malignant tumors. Gene and protein expression of both TFF1 and TFF3 was greater in lymph node-negative tumors, hormone positive tumors, tumors with moderate levels of Ki67 expression, and in grade II tumors. A strong positive correlation was found between TFF1 and TFF3 genes, and the expression of both negatively correlated with Ki67 and the level of tumor histologic diff erentiation. Our results suggest that TFF1 and TFF3, but not TFF2, may have a role in breast tumor pathogenesis and could be used in the assessment of tumor diff erentiation and malignancy.
Abstract (croatian)
Cilj ovoga istraživanja bio je utvrditi razlike u izražaju gena i proteina TFF1, TFF2 i TFF3 u različitim vrstama tumora dojke te ispitati korelacije između gena TFF i vrsta tumora te gena TFF i kliničko-patoloških karakteristika tumora. U studiju su bile uključene 122 ispitanice kojima je kirurški odstranjen tumor dojke. Uzorci su obrađeni metodom qRT-PCR i metodom imunohistokemije. Geni i proteini TFF1 i TFF3 bili su izraženi u tumorima dojke, dok izražaj gena i proteina TFF2 nije otkriven u tumorskom tkivu. TFF1 je bio izraženiji kod dobroćudnih tumora, dok je TFF3 bio izraženiji kod zloćudnih tumora. TFF1 i TFF3 su bili izraženiji u hormonski ovisnim tumorima, tumorima bez metastaza u limfnim čvorovima, tumorima s umjereno visokim izražajem Ki67 i umjereno diferenciranim tumorima. Jaka pozitivna korelacija uočena je između gena TFF1 i TFF3, a oba su negativno korelirala s faktorom Ki67 i stupnjem diferenciranosti tumora. Dobiveni rezultati pokazuju kako bi TFF1 i TFF3 mogli imati ulogu u patogenezi tumora dojke te bi se potencijalno mogli rabiti za određivanje tumorskog statusa i procjenu malignosti tumora.
Keywords (english)
Keywords (croatian)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleActa clinica Croatica
Numberingvol. 57, no. 2, pp. 264-277
p-ISSN0353-9466
e-ISSN1333-9451
DOIhttps://doi.org/10.20471/acc.2018.57.02.06
URN:NBNurn:nbn:hr:239:933099
Publication2018
Document URLhttps://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=304890
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2019-09-26 12:02:53