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Is Shorter Transient Middle Cerebral Artery Occlusion (t-MCAO) Duration Better in Stroke Experiments on Diabetic Female Sprague Dawely rats?
Mišir, Mihael ; Renić, Marija ; Mihalj, Martina ; Novak, Sanja ; Drenjančević, Ines
2016.
izvorni znanstveni rad
AIM: To determine optimal duration of transient middle cerebral artery occlusion (t-MCAO) for a stroke model in female diabetic Sprague-Dawley (SD) rats. METHODS: Streptozotocin-induced type-1 diabetic SD female rats (n = 25, 12 weeks old, five groups; n = 5 per group) were subjected to different duration of t-MCAO (20, 30, 45, 60 and 90 minutes) followed by reperfusion. A control group of rats without diabetes (n = 5) was subjected to 30 minutes of t-MCAO followed by reperfusion. Twenty-four hours after reperfusion, infarct volumes were evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining. RESULTS: Intra-ischaemic reductions of regional cerebral blood flow (rCBF) were similar in all groups (68-75% of baseline values). Reperfusion was significantly impaired in the 90-minute ischaemia group (56-62% vs 80-125% in other groups). Twenty minutes of t-MCAO induced a small infarct (3 ± 5% of ischaemic hemisphere). Thirty minutes of ischaemia produced a significantly larger infarct (46 ± 6%). In the 45 and 60 minute groups, ischaemia infarct was 52 ± 5% and 59 ± 3% of the ischaemic hemisphere, respectively. Ischaemia of 90' led to a massive stroke (89 ± 6% of ischaemic hemisphere encompassing the whole striatum (22 ± 3%) and almost the whole MCA irrigated cortex area (67 ± 6%)). Thirty minutes of t-MCAO did not produce stroke in the control group. CONCLUSION: The diabetic rat stroke model should be different from the non-diabetic, because female type-1 diabetic SD rats are highly sensitive to brain ischaemia and it is necessary to significantly shorten the duration of t-MCAO, optimally to 30 minutes.
Animals Blood glucose/physiology Cerebral infarction/diagnosis Cerebral infarction/etiology Cerebrovascular circulation/physiology Diabetes mellitus, experimental/complications Disease models, animal Fasting Female Infarction, middle cerebral artery/complications Infarction, middle cerebral artery/diagnosis Laser-doppler flowmetry Rats Rats, sprague-dawley Statistics, nonparametric Time factors
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Vrsta sadržaja: text
Jezik sadržaja: engleski
Otvoreni pristup
Autori Mišir, Mihael (Autor) (mbz: 357666) ; Renić, Marija (Autor) (mbz: 194961) ; Mihalj, Martina (Autor) (mbz: 322792) ; Novak, Sanja (Autor) (mbz: 326355) ; Drenjančević, Ines (Autor) (mbz: 209603)
Studijski program
Nema podataka
Umjetnička i znanstvena područja, polja i grane
Biomedicina i zdravstvo / Kliničke medicinske znanosti / interna medicina
Datumi
Datum objave publikacije: 2016.
URL-ovi
https://www.tandfonline.com/doi/full/10.1080/02699052.2016.1195518
Jezici
engleski
Institucije
Sveučilište Josipa Jurja Strossmayera u Osijeku, Sveučilište Josipa Jurja Strossmayera u Osijeku, Medicinski fakultet Osijek
Sveučilište u Zagrebu, Sveučilište u Zagrebu, Medicinski fakultet
Status publikacije
objavljen
Datum izrade zapisa
20.01.2020.
Broj posjeta
0
Broj preuzimanja
0
Identifikatori
DOI: 10.1080/02699052.2016.1195518
URN:NBN: https://urn.nsk.hr/urn:nbn:hr:239:717761
Publikacija
pISSN: 0269-9052
eISSN: 1362-301X
Broj: 30
Sveščić: 11
Početna stranica: 1390
Zadnja stranica: 6